Outlines For Picking Out Central Issues In Osce For Medical

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“We wanted to probe this process in medical interview manchester live animals so we could see the spatiotemporal relationship between pericytes and MMP activation in vivo–in the acute stroke time frame.” To do this, the team combined several novel tools to create a unique study protocol using transgenic mice, two-photon fluorescent microscopy, and a fluorescent gelatin-based reporter of MMPs that only one other research group 8 year medical program interview questions had ever used to study the intact brain. The researchers also used photothrombosis to block blood flow in a small area of the capillary bed and imaged transgenic mouse lines expressing bright fluorescent reporters specifically in the pericytes to clearly identify them. “The successful combination of technologies is certainly one of the innovations of this project,” said Shih. “It’s the first study to combine these tools to look at the relationship among pericytes, MMP activity, and BBB leakage in ischemia.” Their findings revealed that ischemia resulted in extremely rapid (within tens of minutes), localized activation of MMP-9 and plasma leakage. Furthermore, plasma leakage occurred preferentially where the pericyte somata adjoined the capillary wall–not homogeneously along the length of the capillaries as previously imagined by the group. These results provide strong evidence that pericytes–normally protectors of the BBB–contribute to early BBB degradation during ischemic stroke. Using the new technology, the team did not have to extract and cut the brain and so did not lose the structure of the vasculature and blood flow. “We had an intact system and could see where things were coming from and we were very surprised,” said Shih. “I thought, ‘I’ve been looking at this for years and I never knew that there was this beautiful co-localization.’ It told us we were looking at something really interesting.

For the original version including any supplementary images or video, visit https://www.eurekalert.org/pub_releases/2016-12/muos-aio120216.php


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